Zaitsev V, Johnsen U, Reher M, Ortjohann M, Taylor GL, Danson MJ, Schönheit P, Crennell SJ. (2018)
Insights into the Substrate Specificity of Archaeal Entner-Doudoroff Aldolases: The Structures of Picrophilus torridus 2-Keto-3-deoxygluconate Aldolase and Sulfolobus solfataricus 2-Keto-3-deoxy-6-phosphogluconate Aldolase in Complex with 2-Keto-3-deoxy-6-phosphogluconate.
Biochemistry. doi: 10.1021/acs.biochem.8b00535. [Epub ahead of print]

Tästensen JB, Schönheit P. (2018)
Two distinct glyceraldehyde-3-phosphate dehydrogenases in glycolysis and gluconeogenesis in the archaeon Haloferax volcanii.
FEBS Lett. 592(9):1524-1534. doi: 10.1002/1873-3468.13037.


Birkeland NK, Schönheit P, Poghosyan L, Fiebig A, Klenk HP. (2017)
Complete genome sequence analysis of Archaeoglobus fulgidus strain 7324 (DSM 8774), a hyperthermophilic archaeal sulfate reducer from a North Sea oil field.
Stand Genomic Sci. 12:79. doi: 10.1186/s40793-017-0296-5.

Borjian F, Johnsen U, Schönheit P, Berg IA. (2017)
Succinyl-CoA:Mesaconate CoA-Transferase and Mesaconyl-CoA Hydratase, Enzymes of the Methylaspartate Cycle in Haloarcula hispanica.
Front Microbiol. 8:1683. doi: 10.3389/fmicb.2017.01683.

Sutter JM, Johnsen U, Schönheit P. (2017)
Characterization of a pentonolactonase involved in D-xylose and L-arabinose catabolism in the haloarchaeon Haloferax volcanii.
FEMS Microbiol Lett. 364(13). doi: 10.1093/femsle/fnx140.

Antranikian G, Suleiman M, Schäfers C, Adams MWW, Bartolucci S, Blamey JM, Birkeland NK, Bonch-Osmolovskaya E, da Costa MS, Cowan D, Danson M, Forterre P, Kelly R, Ishino Y, Littlechild J, Moracci M, Noll K, Oshima T, Robb F, Rossi M, Santos H, Schönheit P, Sterner R, Thauer R, Thomm M, Wiegel J, Stetter KO. (2017)
Diversity of bacteria and archaea from two shallow marine hydrothermal vents from Vulcano Island.
Extremophiles. 21(4):733-742. doi: 10.1007/s00792-017-0938-y.


Chen X, Schreiber K, Appel J, Makowka A, Fähnrich B, Roettger M, Hajirezaei MR, Sönnichsen FD, Schönheit P, Martin WF, Gutekunst K. (2016)
The Entner-Doudoroff pathway is an overlooked glycolytic route in cyanobacteria and plants.
Proc Natl Acad Sci U S A.;113(19):5441-6. doi: 10.1073/pnas.1521916113.

Sutter JM, Tästensen JB, Johnsen U, Soppa J, Schönheit P. (2016)
Key Enzymes of the Semiphosphorylative Entner-Doudoroff Pathway in the Haloarchaeon Haloferax volcanii: Characterization of Glucose Dehydrogenase, Gluconate Dehydratase, and 2-Keto-3-Deoxy-6-Phosphogluconate Aldolase.
J Bacteriol;198(16):2251-62. doi: 10.1128/JB.00286-16.

Weiße RH, Faust A, Schmidt M, Schönheit P, Scheidig AJ. (2016)
Structure of NDP-forming Acetyl-CoA synthetase ACD1 reveals a large rearrangement for phosphoryl transfer.
Proc Natl Acad Sci U S A. pii: 201518614.

Schönheit P, Buckel W, Martin WF. (2016)
On the Origin of Heterotrophy.
Trends Microbiol. 24(1):12-25.


López Muñoz MM, Schönheit P, Metcalf WW. (2015)
Genetic, Genomic, and Transcriptomic Studies of Pyruvate Metabolism in Methanosarcina barkeri Fusaro.
J Bacteriol. 197(22): 3592-600.

Pickl A, Schönheit P. (2015)
The oxidative pentose phosphate pathway in the haloarchaeon Haloferax volcanii involves a novel type of glucose-6-phosphate dehydrogenase – The archaeal Zwischenferment
FEBS Lett. 589(10): 1105-11.

Nelson-Sathi S, Sousa FL, Roettger M, Lozada-Chávez N, Thiergart T, Janssen A, Bryant D, Landan G, Schönheit P, Siebers B, McInerney JO, Martin WF. (2015)
Origins of major archaeal clades correspond to gene acquisitions from bacteria.
Nature. 517(7532): 77-80.

Johnsen U, Sutter JM, Schulz AC, Tästensen JB, Schönheit P. (2015)
XacR - a novel transcriptional regulator of D-xylose and L-arabinose catabolism in the haloarchaeon Haloferax volcanii.
Environ Microbiol. 17(5): 1663-1673.


Pickl A, Johnsen U, Archer RM, Schönheit P. (2014)
Identification and characterization of 2-keto-3-deoxygluconate kinase and 2-keto-3-deoxygalactonate kinase in the haloarchaeon Haloferax volcanii.
FEMS Microbiol Lett. 361(1): 76-83.


Schmidt M, Schönheit P (2013)
Acetate formation in the photoheterotrophic bacterium Chloroflexus aurantiacus involves an archaeal type ADP-forming acetyl-CoA synthetase isoenzyme I.
FEMS Microbiol Lett. 349: 171-179.

Johnsen U, Sutter JM, Zaiß H, Schönheit P (2013)
L-Arabinose degradation pathway in the haloarchaeon Haloferax volcanii involves a novel type of L-arabinose dehydrogenase.
Extremophiles. 17: 897-909.

Solomons JT, Johnsen U, Schönheit P, Davies C (2013)
3-Phosphoglycerate is an allosteric activator of pyruvate kinase from the hyperthermophilic archaeon Pyrobaculum aerophilum.
Biochemistry. 52: 5865-75.

Mukhopadhyay B, Hansen T, Schönheit P, Hasson MS, Sanders DA (2013)
Atomic structure of a hyperthermophilic acetate kinase.
In: Acetate. Versatile building block of biology and chemistry. D.A. Sanders (Ed.) Nova Science Publishers. Inc. New York, pp. 15-28. No abstract available.


Pickl A, Johnsen U, Schönheit P (2012)
Fructose degradation in the haloarchaeon haloferax volcanii involves a bacterial type phosphoenolpyruvate-dependent phosphotransferase system, fructose-1-phosphate kinase, and class ii fructose-1,6-bisphosphate aldolase.
J Bacteriol. 194: 3088-97.


Nunn CE, Johnsen U, Schönheit P, Fuhrer T, Sauer U, Hough DW, Danson MJ (2010)
Metabolism of pentose sugars in the hyperthermophilic archaea Sulfolobus solfataricus and Sulfolobus acidocaldarius.
J Biol Chem. 285: 33701-9.

Reher M, Fuhrer T, Bott M, Schönheit P (2010)
The nonphosphorylative Entner-Doudoroff pathway in the thermoacidophilic euryarchaeon Picrophilus torridus involves a novel 2-keto-3-deoxygluconate- specific aldolase.
J Bacteriol. 192: 964-74.


Johnsen U, Dambeck M, Zaiss H, Fuhrer T, Soppa J, Sauer U, Schönheit P. (2009)
D-xylose degradation pathway in the halophilic archaeon Haloferax volcanii.
J Biol Chem. 284: 27290-303.


Karlsson EN, Labes A, Turner P, Fridjonsson OH, Wennerberg C, Pozzo T, Hreggvidson GO, Kristjansson JK, Schönheit P (2008)
Differences and similarities in enzymes from the neopullulanase subfamily isolated from thermophilic species
Biologia. 63: 1006-1014. No abstract available.

Bräsen C, Schmidt M, Grötzinger J, Schönheit P. (2008)
Reaction mechanism and structural model of ADP-forming Acetyl-CoA synthetase from the hyperthermophilic archaeon Pyrococcus furiosus: Evidence for a second active site histidine residue.
Journal Biol Chem. 283: 15409-18.

Labes A, Karlsson EN, Fridjonsson OH, Turner P, Hreggvidson GO, Kristjansson JK, Holst O, Schönheit P. (2008)
Isolation of new members of glycoside hydrolase family 13 derived from environmental DNA.
Appl Environ Microbiol. 74: 1914-21

Schönheit P. (2008)
Glycolysis in Hyperthermomphiles (Chapter 7).
In 'Thermophiles - Biology and Technology at High Temperatures'. Robb F, Antranikan G, Grogan D, Driessen A (eds.), CRC Press Taylor & Francis Group, Boca Raton London New York. pp 99-112. No abstract available.


Labes A, Schönheit P. (2007)
Unusual starch degradation pathway via cyclodextrins in the hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus strain 7324.
J Bacteriol. 189: 8901-13.

Johnsen U, Schönheit P. (2007)
Characterization of cofactor dependent and cofactor independent phosphoglycerate mutase from archaea.
Extremophiles 11: 647-57.

Reher M, Gebhard S, Schönheit P. (2007)
Glyceraldehyde-3-phosphate ferredoxin oxidoreductase (GAPOR) and nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN), key enzymes of the respective modified Embden-Meyerhof pathways in the hyperthermophilic crenarchaeota Pyrobaculum aerophilum and Aeropyrum pernix.
FEMS Microbiol Lett 273: 196-205.

Hansen T, Arnfors L, Ladenstein R, Schönheit P. (2007)
The phosphofructokinase-B (MJ0406) from Methanocaldococcus jannaschii represents a nucleoside kinase with a broad substrate specificity.
Extremophiles 11: 105-14.


L'Haridon S, Reysenbach AL, Tindall BJ, Schönheit P, Banta A, Johnsen U, Schumann P, Gambacorta A, Stackebrandt E, Jeanthon C. (2006)
Desulfurobacterium atlanticum sp. nov., Desulfurobacterium pacificum sp. nov., and Thermovibrio guaymasensis sp. nov., three thermophilic members of the Desulfurobacteriaceae fam. nov., a deep branching lineage within the Bacteria.
Int. J. Syst. Evol. Microbiol. 56: 2843-2852.

Arnfors L, Hansen T, Schönheit P, Ladenstein R, Meining W. (2006)
Crystal structure of Methanocaldococcus jannaschii nucleoside kinase - an archeal member of the ribokinase family.
Acta Cryst. D 62: 1085-1097.

Reher M, Bott M, Schönheit P. (2006)
Characterization of glycerate kinase (2-phosphoglycerate forming), a key enzyme of the nonphosphorylative Entner-Doudoroff pathway, from the thermoacidophilic euryarcheon Picrophilus torridus.
FEMS Microbiol. Lett. 259: 113-119.

Reher M, Schönheit P. (2006)
Glyceraldehyde dehydrogenases from the thermoacidophilic euryarchaeota Picrophilus torridus and Thermoplasma acidophilum, key enzymes of the non-phosphorylative Entner-Doudoroff pathway, constitute a novel enzyme family within the aldehyde dehydrogenase superfamily.
FEBS Lett. 580: 1198-204.

Leben am Siedepunkt
Uni-Zeit vom 28. Januar 2006


Hansen T, Schlichting B, Grotzinger J, Swan MK, Davies C, Schönheit P. (2005)
Mutagenesis of catalytically important residues of cupin type phosphoglucose isomerase from Archaeoglobus fulgidus.
FEBS J. 272: 6266-75.

Siebers B, Schönheit P. (2005)
Unusual pathways and enzymes of central carbohydrate metabolism in Archaea.
Curr. Opin. Microbiol. 8: 695-705. Review.

Bräsen C, Schönheit P. (2005)
AMP-forming acetyl-CoA synthetase from the extremely halophilic archaeon Haloarcula marismortui: purification, identification and expression of the encoding gene, and phylogenetic affiliation.
Extremophiles 9: 355-65.

Turner P, Labes A, Fridjonsson OH, Hreggvidson GO, Schönheit P, Kristjansson JK, Holst O, Karlsson EN. (2005)
Two novel cyclodextrin-degrading enzymes isolated from thermophilic bacteria have similar domain structures but differ in oligomeric state and activity profile.
J Biosci Bioeng. 100: 380-90.

Hansen T, Schönheit P. (2005)
Escherichia coli phosphoglucose isomerase can be substituted by members of the PGI family, the PGI/PMI family, and the cPGI family.
FEMS Microbiol. Lett. 250: 49-53.

Arnfors L, Hansen T, Meining W, Schönheit P, Ladenstein R. (2005)
Expression, purification, crystallization and preliminary X-ray analysis of a nucleoside kinase from the hyperthermophile Methanocaldococcus jannaschii.
Acta Cryst. F 61: 591-4.

Hansen T, Schlichting B, Felgendreher M, Schönheit P. (2005)
Cupin-type phosphoglucose isomerases (Cupin-PGIs) constitute a novel metal-dependent PGI family representing a convergent line of PGI evolution.
J. Bacteriol. 187: 1621-31.

Lehtiö L, Fabrichniy I, Hansen T, Schönheit P, Goldman A. (2005)
Unusual twinning in an acetyl coenzyme A synthetase (ADP-forming) from Pyrococcus furiosus.
Acta Cryst. D 61: 350-4.

Bräsen C, Urbanke C, Schönheit P. (2005)
A novel octameric AMP-forming acetyl-CoA synthetase from the hyperthermophilic crenarchaeon Pyrobaculum aerophilum.
FEBS Lett. 579: 477-82.


Bräsen C, Schönheit P. (2004)
Regulation of acetate and acetyl-CoA converting enzymes during growth on acetate and/or glucose in the halophilic archaeon Haloarcula marismortui.
FEMS Microbiol. Lett. 241: 21-6.

Hansen T, Urbanke C, Schönheit P. (2004)
Bifunctional phosphoglucose/phosphomannose isomerase from the hyperthermophilic archaeon Pyrobaculum aerophilum.
Extremophiles 8: 507-12.

Swan MK, Hansen T, Schönheit P, Davies C. (2004)
Structural basis for phosphomannose isomerase activity in phosphoglucose isomerase from Pyrobaculum aerophilum: a subtle difference between distantly related enzymes.
Biochemistry 43: 14088-95.

Bräsen C, Schönheit P. (2004)
Unusual ADP-forming acetyl-coenzyme A synthetases from the mesophilic halophilic euryarchaeon Haloarcula marismortui and from the hyperthermophilic crenarchaeon Pyrobaculum aerophilum.
Arch. Microbiol. 182: 277-87.

Johnsen U, Schönheit P. (2004)
Novel xylose dehydrogenase in the halophilic archaeon Haloarcula marismortui.
J. Bacteriol. 186: 6198-207.

Swan MK, Hansen T, Schönheit P, Davies C. (2004)
A novel phosphoglucose isomerase (PGI)/phosphomannose isomerase from the crenarchaeon Pyrobaculum aerophilum is a member of the PGI superfamily: structural evidence at 1.16-A resolution.
J. Biol. Chem. 279: 39838-45.

Swan MK, Hansen T, Schönheit P, Davies C. (2004)
Crystallization and preliminary X-ray diffraction analysis of phosphoglucose/phosphomannose isomerase from Pyrobaculum aerophilum.
Acta Cryst. D 60: 1481-3.

Hansen T, Schönheit P. (2004)
ADP-dependent 6-phosphofructokinase, an extremely thermophilic, non-allosteric enzyme from the hyperthermophilic, sulfate-reducing archaeon Archaeoglobus fulgidus strain 7324.
Extremophiles 8: 29-35.

Rudolph B, Hansen T, Schönheit P. (2004)
Glucose-6-phosphate isomerase from the hyperthermophilic archaeon Methanococcus jannaschii: characterization of the first archaeal member of the phosphoglucose isomerase superfamily.
Arch. Microbiol. 181: 82-7.

Hansen T, Wendorff D, Schönheit P. (2004)
Bifunctional phosphoglucose/phosphomannose isomerases from the Archaea Aeropyrum pernix and Thermoplasma acidophilum constitute a novel enzyme family within the phosphoglucose isomerase superfamily.
J. Biol. Chem. 279: 2262-72.


Swan MK, Solomons JT, Beeson CC, Hansen T, Schönheit P, Davies C. (2003)
Structural evidence for a hydride transfer mechanism of catalysis in phosphoglucose isomerase from Pyrococcus furiosus.
J. Biol. Chem. 278: 47261-8.

Hansen T, Schönheit P. (2003)
ATP-dependent glucokinase from the hyperthermophilic bacterium Thermotoga maritima represents an extremely thermophilic ROK glucokinase with high substrate specificity.
FEMS Microbiol. Lett. 226: 405-11.

Labes A, Schönheit P. (2003)
ADP-dependent glucokinase from the hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus strain 7324.
Arch. Microbiol. 180:69-75.

Johnsen U, Hansen T, Schönheit P. (2003)
Comparative analysis of pyruvate kinases from the hyperthermophilic archaea Archaeoglobus fulgidus, Aeropyrum pernix, and Pyrobaculum aerophilum and the hyperthermophilic bacterium Thermotoga maritima: unusual regulatory properties in hyperthermophilic archaea.
J. Biol. Chem. 278: 25417-27.


Hansen T, Schlichting B, Schönheit P. (2002)
Glucose-6-phosphate dehydrogenase from the hyperthermophilic bacterium Thermotoga maritima: expression of the g6pd gene and characterization of an extremely thermophilic enzyme.
FEMS Microbiol. Lett. 216: 249-53.

Hansen T, Reichstein B, Schmid R, Schönheit P. (2002)
The first archaeal ATP-dependent glucokinase, from the hyperthermophilic crenarchaeon Aeropyrum pernix, represents a monomeric, extremely thermophilic ROK glucokinase with broad hexose specificity.
J. Bacteriol. 184: 5955-65.

Hansen T, Musfeldt M, Schönheit P. (2002)
ATP-dependent 6-phosphofructokinase from the hyperthermophilic bacterium Thermotoga maritima: characterization of an extremely thermophilic, allosterically regulated enzyme.
Arch. Microbiol. 177: 401-9.

Musfeldt M, Schönheit P. (2002)
Novel type of ADP-forming acetyl coenzyme A synthetase in hyperthermophilic archaea: heterologous expression and characterization of isoenzymes from the sulfate reducer Archaeoglobus fulgidus and the methanogen Methanococcus jannaschii.
J. Bacteriol. 184: 636-44.


Hansen T, Schönheit P. (2001)
Sequence, expression, and characterization of the first archaeal ATP-dependent 6-phosphofructokinase, a non-allosteric enzyme related to the phosphofructokinase-B sugar kinase family, from the hyperthermophilic crenarchaeote Aeropyrum pernix.
Arch. Microbiol. 177: 62-9.

Labes A, Schönheit P. (2001)
Sugar utilization in the hyperthermophilic, sulfate-reducing archaeon Archaeoglobus fulgidus strain 7324: starch degradation to acetate and CO2 via a modified Embden-Meyerhof pathway and acetyl-CoA synthetase (ADP-forming).
Arch. Microbiol. 176: 329-38. Erratum in: Arch. Microbiol. 177: 431-2. (2002)

Hansen T, Oehlmann M, Schönheit P. (2001)
Novel type of glucose-6-phosphate isomerase in the hyperthermophilic archaeon Pyrococcus furiosus.
J. Bacteriol. 183: 3428-35.

Bräsen C, Schönheit P. (2001)
Mechanisms of acetate formation and acetate activation in halophilic archaea.
Arch. Microbiol. 175: 360-8. Erratum in: Arch. Microbiol. 180: 504. (2003)

Johnsen U, Selig M, Xavier KB, Santos H, Schönheit P. (2001)
Different glycolytic pathways for glucose and fructose in the halophilic archaeon Halococcus saccharolyticus.
Arch. Microbiol. 175: 52-61. Erratum in: Arch. Microbiol. 180: 503. (2003)

Schönheit P. (2001)
Phosphate acetyltransferase and acetate kinase from Thermotoga maritima.
Methods Enzymol. 331: 168-76. No abstract available.


Hansen T, Schönheit P. (2000)
Purification and properties of the first-identified, archaeal, ATP-dependent 6-phosphofructokinase, an extremely thermophilic non-allosteric enzyme, from the hyperthermophile Desulfurococcus amylolyticus.
Arch. Microbiol. 173: 103-9.


Musfeldt M, Selig M, Schönheit P. (1999)
Acetyl coenzyme A synthetase (ADP forming) from the hyperthermophilic Archaeon pyrococcus furiosus: identification, cloning, separate expression of the encoding genes, acdAI and acdBI, in Escherichia coli, and in vitro reconstitution of the active heterotetrameric enzyme from its recombinant subunits.
J. Bacteriol. 181: 5885-8.

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