Z2-Project: Enrichment and isolation of Prokaryotes

Z2-Project: Enrichment and isolation of Prokaryotes

This project within the CRC1182 represents a central platform for enrichment, isolation and identification of microorganisms associated with animal and plant metaorganisms studied within the Collaborative research center (CRC) 1182. In general 99 % of microorganisms present in natural environments are currently not cultivable with conventional approaches due to difficulties and limitations associated with cultivation techniques. Thus, cultured microorganisms do not reflect the functional and phylogenetic diversity present within a natural habitat. However, continuous efforts have been invested into improving efficiency of microbial cultivation techniques. Significant progress has been achieved, e.g. by simulation of the natural conditions, refinement of the culture media and culturing microbial consortia. Furthermore, several DNA-based molecular methods, the so called ‘metagenomics’, have been developed in order to explore the diversity of microbial communities by analyzing the complex genomes. Primarily employed to study non-cultivable microbiota for a better understanding of global microbial ecology, metagenomics data also provide information on the functional role of the different microbes. A recent addition to the omics repertoire is single-cell genomics, which has advanced the field of microbiology from the analysis of microbial metagenomes by recognizing each microbial cell as a separate and unique entity.

Web-pages of Origin and Function of Metaorganisms - Z2: Microorganism: Culture and single cell analysis