Prasse D, Förstner KU, Jäger D, Rolf Backofen, Schmitz RA
sRNA154 a newly identified regulator of nitrogen fixation in Methanosarcina mazei strain Gö1.
RNA Biology. accepted.

Weiland-Bräuer N, Langfeldt D, Schmitz RA
Construction and Screening of Marine Metagenomic Large Insert Libraries.
Methods Mol Biol. 1539:23-42.


Cassidy L, Prasse D, Linke D, Schmitz RA, Tholey A
Combination of Bottom-up 2D-LC-MS and Semi-top-down GelFree-LC-MS Enhances Coverage of Proteome and Low Molecular Weight Short Open Reading Frame Encoded Peptides of the Archaeon Methanosarcina mazei.
J Proteome Res. 15(10):3773-3783.

Martínez-Pérez C, Mohr W, Löscher CR, Dekaezemacker J, Littmann S, Yilmaz P, Lehnen N, Fuchs BM, Lavik G, Schmitz RA, LaRoche J, Kuypers MM.
The small unicellular diazotrophic symbiont, UCYN-A, is a key player in the marine nitrogen cycle.
Nature Microbiology. 16163. doi: 10.1038/nmicrobiol.2016.163.

Dar D, Prasse D, Schmitz RA, Sorek R
Widespread formation of alternative 3′ UTR isoforms via transcription termination in archaea
Nature Microbiology. 16143. doi:10.1038/nmicrobiol.2016.143

Fischer MA, Güllert S, Neulinger SC, Streit WR, Schmitz RA
Evaluation of 16S rRNA gene primer pairs for monitoring microbial community structures showed high reproducibility within and low comparability between datasets generated with multiple archaeal and bacterial primer pairs
Frontiers Microbiology. 7: 1297. doi:  10.3389/fmicb.2016.01297

Malek I, Schaber CF, Heinlein T, Schneider JJ, Gorb SN, Schmitz RA
Vertically aligned multi Walled Carbon Nanotubes prevent Biofilm Formation of Medically Relevant Bacteria
Journal of Materials Chemistry B in press

Weiland-Bräuer N, Kisch M, Pinnow N, Liese A, Schmitz RA
Highly effective inhibition of biofilm formation by the first metagenome-derived AI-2 quenching enzyme
Frontiers in Microbiology. 7:1098. doi: 10.3389/fmicb.2016.01098

Güllert S, Fischer MA, Turaev D, Noebauer B, Ilmberger N, Wemheuer B, Alawi M, Rattei T, Daniel R, Schmitz RA, Grundhoff A, Streit WR
Deep metagenome and metatranscriptome analyses of microbial communities affiliated with an industrial biogas fermenter, a cow rumen, and elephant feces reveal major differences in carbohydrate hydrolysis strategies
Biotechnology for Biofuels. 9:121 doi: 10.1186/s13068-016-0534-x

Mensch B, Neulinger SC, Graiff A, Pansch A, Künzel S, Fischer MA, Schmitz RA
Restructuring of epibacterial communities on Fucus vesiculosus forma mytili in response to elevated pCO2 and increased temperature levels.
Frontiers in Microbiology. 7:434. doi: 10.3389/fmicb.2016.00434

Arévalo-Martínez DL, Kock A, Löscher CR, Schmitz RA, Stramma L, Bange HW
Influence of mesoscale eddies on the distribution of nitrous oxide in the eastern tropical South Pacific.
Biogeosciences, 13, 1105-1118, doi:10.5194/bg-13-1105-2016

Kern T, Fischer MA, Deppenmeier U, Schmitz RA, Rother M
Methanosarcina flavescens sp. nov., a methanogenic archaeon isolated from a full-scale anaerobic digester.
Int J Syst Evol Microbiol. 2016 Jan 12. doi: 10.1099/ijsem.0.000894. [Epub ahead of print]


Meyer J, Löscher CR, Neulinger SC, Reichel AF, Loginova A, Borchard C, Schmitz RA, Hauss H, Kiko R, Riebesell U
Changing nutrient stoichiometry affects phytoplankton production, DOP build up and dinitrogen fixation – a mesocosm experiment in the eastern tropical North Atlantic.
Biogeosciences, 13, 781-794

Löscher CR, Fischer MA, Neulinger SC, Fiedler B, Philippi M, Schütte F, Singh A, Hauss H, Karstensen J, Körtzinger A, Künzel S, Schmitz RA
Hidden biosphere in an oxygen-deficient Atlantic open ocean eddy: future implications of ocean deoxygenation on primary production in the eastern tropical North Atlantic.
Biogeosciences, 12, 7467-7482.

Kalvelage T, Lavik G, Jensen MM, Revsbech NP, Löscher C, Schunck H, Desai DK, Hauss H, Kiko R, Holtappels M, LaRoche J, Schmitz RA, Graco MI, Kuypers MM
Aerobic Microbial Respiration In Oceanic Oxygen Minimum Zones.
PLoS One. 10(7):e0133526.

Midekssa, MJ, Löscher, CR, Schmitz, RA, Assefa F
Characterization of phosphate solubilizing rhizobacteria isolated from lentil growing areas of Ethiopia.
Afr. J. Microbiol. Res. 9:1637-1648.

Weiland-Bräuer N, Neulinger SC, Pinnow N, Künzel S, Baines JF, Schmitz RA
Changing company: Composition of bacterial communities associated with Aurelia aurita changes with compartment, life stage and population.
Applied environmental microbiology. 81(17):6038-52.

Arévalo-Martínez DL, Kock A, Löscher CR, Schmitz RA, Bange HW
Evidence of massive nitrous oxide emissions from the tropical South Pacific Ocean.
Nature Geoscience,
8: 530–533.

Heinsen FA, Knecht H, Neulinger SC, Schmitz RA, Däumer C, Kühbacher T, Rosenstiel PC, Schreiber S, Friedrichs AK, Ott SJ
Dynamic changes of the luminal and mucosa-associated gut microbiota during and after antibiotic therapy with paromomycin.
Gut microbes,

Prasse D, Thomsen J, DeSantis R, Muntel J, Becher D, Schmitz RA
First description of small proteins encoded by spRNAs in Methanosarcina mazei strain Gö1.
Biochimie, 117:138-48.

Bang C, Schmitz RA
Archaea associated with human surfaces: not to be underestimated.
FEMS Microbiology reviews, 39(5):631-48.

Bauersachs T, Weidenbach K, Schmitz RA, Lorenz Schwark L
Distribution of glycerol ether lipids in halophilic, methanogenic and hyperthermophilic archaea.
Organic Geochemistry, 83–84: 101–108.

Löscher CR, LaRoche J, Schmitz RA
Nitrogen fixation.
ed. DeBruin, CHAPTER 105: Underestimation of marine dinitrogen fixation: A novel method and novel diazotrophic habitats. Review. No abstract available.


Weiland-Bräuer N, Pinnow N, Schmitz RA
Novel reporter for identification of interference with AHL and autoinducer-2 quorum sensing..
Applied and environmental Microbiology. 81(4):1477-89.

Langfeldt D, Neulinger SC, Stiesch M, Stumpp N, Bang C, Schmitz RA, Eberhard J
Health- and disease-associated species clusters in complex natural biofilms determine the innate immune response in oral epithelial cells during biofilm maturation.
FEMS Microbiol Lett. 360(2):137-43.

Bang C, Ehlers C, Orell A, Prasse D, Spinner M, Gorb SN, Albers SV, Schmitz RA
Biofilm formation of mucosa-associated methanoarchaeal strains.
Front. Microbiol. 5:353.

Weidenbach K, Ehlers C, Schmitz RA
The transcriptional activator NrpA is crucial for inducing nitrogen fixation in Methanosarcina mazei Gö1 under nitrogen limitation.
FEBS J. 281(15):3507-22.

Bang C, Weidenbach K, Gutsmann T, Heine H, Schmitz RA
The intestinal Archaea Methanosphaera stadtmanae and Methanobrevibacter smithii Activate human dendritic cells.
PLoS One. 9(6):e99411.

Löscher CR, Großkopf T, Desai FD, Gill D, Schunck H, Croot PL, Schlosser C, Neulinger SC, Pinnow N, Lavik G, Kuypers MM, LaRoche J, Schmitz RA
Facets of diazotrophy in the oxygen minimum zone waters off Peru.
ISME J.  8(11):2180-92.

Knecht H, Neulinger SC, Heinsen FA, Knecht C, Schilhabel A, Schmitz RA, Zimmermann A, dos Santos VM, Ferrer M, Rosenstiel PC, Schreiber S, Friedrichs AK, Ott SJ
Effects of β-lactam antibiotics and fluoroquinolones on human gut microbiota in relation to Clostridium difficile associated diarrhea.
PLoS One. 9(2):e89417.

Brendel J, Stoll B, Lange SJ, Sharma K, Lenz C, Stachler A, Maier L, Richter H, Nickel L, Schmitz RA, Randau L, Urlaub H, Backofen R, Marchfelder A
A complex of Cas proteins 5, 6, and 7 is required for the biogenesis and stability of crRNAs in Haloferax volcanii.
JBC. 289: 7164-7177.

Susanti D, Wong JH, Vensel WH, Loganathan U, DeSantis R, Schmitz RA, Balsera M, Buchanan BB, Mukhopadhyay B
Thioredoxin targets fundamental processes in a methane-producing archaeon, Methanocaldococcus jannaschii.
Proc Natl Acad Sci U S A. 111(7): 2608-13.

Langfeldt D, Neulinger SC, Heuer W, Staufenbiel I, Künzel S, Baines JF, Eberhard J, Schmitz RA
Composition of microbial oral biofilms during maturation in young healthy adults.
PLoS One. 9(2): e87449.


Schunck H, Lavik G, Desai DK, Großkopf T, Kalvelage T, Löscher CR, Paulmier A, Contreras S, Siegel H, Holtappels M, Rosenstiel P, Schilhabel MB, Graco M, Schmitz RA, Kuypers MM, Laroche J
Giant hydrogen sulfide plume in the oxygen minimum zone off Peru supports chemolithoautotrophy.
PLoS One. 8(8): e68661.

Kalvelage T, Lavik G, Lam P, Contreras S, Arteaga L, Löscher C, Oschlies A, Paulmier A, Stramma L, Kuypers M
Nitrogen cycling driven by organic matter export in the South Pacific oxygen minimum zone.
Nature Geoscience. 6:228–234.

Nickel L, Weidenbach K, Jäger D, Backofen R, Lange SJ, Heidrich N, Schmitz RA
Two CRISPR-Cas systems in Methanosarcina mazei strain Gö1 display common processing features despite belonging to different types I and III.
RNA Biology. 10(5): 779-91.

Bertics VJ, Löscher C, Salonen I, Schmitz RA, Treude T
Occurrence of benthic microbial nitrogen fixation, coupled to sulfate reduction, and denitrification in the seasonally hypoxic Eckernförde Bay, Baltic Sea.
Biogeosciences. 10: 1243-1258.

Eberhard J , Grote K, Luchtefeld M, Heuer W, Schuett H, Divchev D, Scherer R, Schmitz-Streit R, Langfeldt D, Stumpp N, Staufenbiel I, Schieffer B, Stiesch M
Experimental Gingivitis Induces Systemic Inflammatory Markers in Young Healthy Individuals: A Single-Subject Interventional Study.
PLOS one. 8(2): e55265.

Prasse D, Ehlers C, Backofen R, Schmitz RA
Regulatory RNAs in archaea: first target identification in Methanoarchaea.
Biochem Soc Trans. 41(1): 344-9.

Balsera M, Uberegui E, Dwi S, Schmitz RA, Mukhopadhyay B, Schürmann P, Buchanan BB
Ferredoxin-thioredoxin reductase (FTR) links the regulation of oxygenic photosynthesis to deeply rooted bacteria.
Planta. 237(2): 619-35.


Jäger D, Pernitzsch SR, Richter A, Backofen R, Sharma CM, Schmitz RA
An archaeal sRNA targeting cis- and trans-encoded mRNAs via two distinct domains.
Nucleic Acid Research. 40(21): 10964-79.

Großkopf T, Mohr W, Baustian T, Schunck H, Gill D, Kuypers MM, Lavik G, Schmitz RA, Wallace DWR, LaRoche L
Doubling of marine N2 fixation rates based on direct measurements.
NATURE. 488(7411): 361-4.

Ilmberger N, Meske D, Juergensen J, Schulte M, Barthen P, Rabausch U, Angelov A, Mientus M, Liebl W, Schmitz RA, Streit WR
Metagenomic cellulases highly tolerant in the presence of ionic liquids - linking thermostability and halotolerance.
Applied microbiology and biotechnology. 95(1): 135-46.

Marchfelder A, Fischer S, Brendel J, Stoll B, Maier L-K, Jäger D, Prasse D, Plagens A, Schmitz R A, Randau L
Small RNAs for Defence and Regulation in Archaea.
Extremophiles. 16(5): 685-96.

Löscher C, Kock A, Könneke M, LaRoche J, Bange HW, Schmitz RA
Evidence for oceanic nitrous oxide production by ammonia-oxidizing archaea.
Biogeosciences. 9: 2419-2429.

Bang C, Schilhabel A, Weidenbach K, Kopp A, Goldmann T, Gutsmann T, Schmitz RA
Effects of Antimicrobial Peptides on Methanogenic Archaea.
Antimicrob Agents Chemother. 56(8): 4123-30.


Schmitz RA, Jäger D, Jellen-Ritter A, Babski J, Soppa J, Marchfelder A
Archaea employ small RNA as regulators.
In Regulatory RNAs in Prokaryotes (Hess, W.R. and Marchfelder, A. eds.), Springer, Vienna. No abstract available.

Thomas F, Diaz-Bone R, Würfel O, Huber B, Weidenbach K, Schmitz RA, Hensel R
Connection between multimetal(loid) methylation in methanoarchaea and central intermediates of methanogenesis.
Appl. Environ Microbiol. 77(24): 8669-8675.

Ehlers C, Jäger D, Schmitz, RA
Establishing a markerless genetic exchange system for Methanosarcina mazei strain Gö1 for constructing chromosomal mutants of small RNA genes
Archaea. 439608. Epub 2011 Sep 20.

Egert M, Schmidt I, Höhne H-M, Lachnit T, Schmitz RA, Breves R
Ribosomal RNA-based profiling of bacteria in the axilla of healthy males suggests right-left asymmetry in bacterial activity
FEMS Microbiology Ecology. 77: 146-53.

Lachnit T, Meske D, Wahl M, Harder T, Schmitz RA.
Epibacterial community patterns on marine macroalgae are host-specific but temporally variable
Environmental Microbiology. 13(3): 655-65.

Milenkov M, Thummer R, Glöer J, Grötzinger J, Jung S, Schmitz RA
Insights into membrane association of Klebsiella pneumoniae NifL under N2 - fixing conditions by mutational analysis
J Bacteriology. 193(3): 695-705.


Barz M, Beimgraben C, Staller T, Germer F, Opitz F, Marquardt C, Schwarz C, Gutekunst C, Vanselow KH, Schmitz RA, LaRoche J, Schulz R, Appel J.
Distribution analysis of hydrogenases in surface waters of marine and freshwater environments
PLos ONE. 5(11): e13846.

Weidenbach K, Ehlers C, Kock J, Schmitz RA.
NrpRII mediates contacts between NrpRI and general transcription factors in the archaeon Methanosarcina mazei Gö1
FEBS J. 277: 4398-4411.

Weiland N, Löscher C, Metzger R, Schmitz RA.
Construction and screening marine metagenomic libraries.
Methods Mol Biol. 668: 51-65.


Jäger D, Sharma CM, Thomsen J, Ehlers C, Vogel J, Schmitz RA.
Deep sequencing analysis of the Methanosarcina mazei Gö1 transcriptome in response to nitrogen availability
Proc Natl Acad Sci U S A. 106(51): 21878-82

Schmeisser C, Liesegang H, Krysciak D, Bakkou N, Le Quéré A, Wollherr A, Heinemeyer I, Morgenstern B, Pommerening-Röser A, Flores M, Palacios R, Brenner S, Gottschalk G, Schmitz RA, Broughton WJ, Perret X, Strittmatter AW, Streit WR.
Rhizobium sp. NGR234 possesses a remarkable number of secretion systems
Appl Environ Microbiol. 75(12): 4035-45.


Glöer J, Thummer R, Ullrich H, Schmitz RA.
Towards understanding the nitrogen signal transduction for nif gene expression in Klebsiella pneumoniae
FEBS J. 275(24): 6281-94.

Weidenbach K, Ehlers C, Kock J, Ehrenreich A, Schmitz RA.
Insights into the NrpR regulon in Methanosarcina mazei Gö1
Archives of Microbiology. 190(3): 319-32.

Weidenbach K, Glöer J, Ehlers C, Sandman K, Reeve JN, Schmitz RA.
Deletion of the archaeal histone in Methanosarcina mazei Gö1 results in reduced growth and genomic transcription.
Mol Microbiol. 67(3): 662-71.


Urban JH, Papenfort K, Thomsen J, Schmitz RA, Vogel J.
A Conserved Small RNA Promotes Discoordinate Expression of the glmUS Operon mRNA to Activate GlmS Synthesis.
J Mol Biol. 373(3): 521-8.

Thummer R, Klimmek O, Schmitz RA.
Biochemical Studies of Klebsiella pneumoniae NifL Reduction Using Reconstituted Partial Anaerobic Respiratory Chains of Wolinella succinogenes.
J Biol Chem. 282(17): 12517-12526.


Veit K, Ehlers C, Ehrenreich A, Salmon K, Hovey R, Gunsalus RP, Deppenmeier U, Schmitz RA
Global transcriptional analysis of Methanosarcina mazei strain Gö1 under different nitrogen availabilities.
Molecular Genetics and Genomics. 276(1): 41-55.


Reeve JN, Schmitz RA
Biology, biochemistry and the molecular machinery of Archaea.
Curr Opin Microbiol. 8(6): 627-9.

Veit K, Ehlers C, Schmitz RA
Effects of nitrogen and carbon sources on transcription of soluble methyltransferases in Methanosarcina mazei strain Gö1.
J. Bacteriology. 187: 6147-6154.

Ehlers C, Weidenbach K, Veit K, Deppenmeier U, Metcalf W, Schmitz RA
Development of genetic methods and construction of chromosomal glnK1 mutant in Methanosarcina mazei strain Gö1.
Molecular Genetics and Genomics. 273: 290-298.

Ehlers C, Weidenbach K, Veit K, Forchhammer K, Schmitz RA
Unique mechanistic features of post translational regulation of glutamin synthetase activity in Methanosarcina mazei in response to nitrogen availability.
Molecular Microbiology. 55: 1841-1854.


Streit WR, Schmitz RA
Metagenomics - the key to the uncultured microbes.
Current Opinion in Microbiology. 7: 492 - 498.

Stips J, Thummer R, Neumann M, Schmitz RA
GlnK effects complex formation between NifA and NifL in Klebsiella pneumoniae.
European Journal of Biochemistry. 271: 3379-3388.

Schmitz RA, Achebach S, Unden G
Analysis of Fumarate nitrate reductase regulator as oxygen sensor in Escherichia coli. In 'Methods in Enzymology, Volume 381, Oxygen sensing'.
Sen CK & Semenza GL (eds.), Academic press Inc., New York. pp 628. No abstract available.

Streit WR, Schmitz RA, Perret X, Staehelin C, Deakin WJ, Raasch C, Liesegang H, Broughton WJ
An Evolutionary Hot Spot: the pNGR234b Replicon of Rhizobium sp. Strain NGR234.
J. Bacteriology.186: 535 - 542.


Schmeisser C, Stöckigt C, Raasch C, Wingender J, Timmis KN, Wenderoth DF, Flemming H-C, Liesegang H, Schmitz RA, Jaeger K-E, Streit WR
Metagenome Survey of Biofilms in Drinking Water Networks.
Appl. Environ. Microbiol. 69: 7298-7309.

Grabbe R, Schmitz RA.
Oxygen control of nif gene expression in Klebsiella pneumoniae depends on NifL reduction at the cytoplasmic membrane by electrons derived from the reduced quinone pool.
Eur J Biochem. 270(7): 1555-66.


Deppenmeier U, Schmitz RA, Gunsalus RP, Fritz HJ, Gottschalk G.
The genome of Methanosarcina mazei: evidence for lateral gene transfer between bacteria and archaea.
J Mol Microbiol Biotechnol. 4(4): 453-61

Schmitz RA, Klopprogge K, Grabbe R, Stips J
Regulation of nitrogen fixation in Klebsiella pneumoniae and Azotobacter vinelandii: NifL, transducing two environmental signals to the nif transcriptional activator NifA .
In 'Regulatory networks in Prokaryotes' Dürre P & Friedrich B (eds.), p. 65-72, Horizon Scientific press, Norfolk. No abstract available.

Schmitz RA, Klopprogge K, Grabbe R
Regulation of nitrogen fixation in Klebsiella pneumoniae and Azotobacter vinelandii: NifL, transducing two environmental signals to the nif transcriptional activator NifA (Minireview).
J. Mol. Microbiol. Biotechnol. 4: 235-242.

Klopprogge K, Grabbe R, Hoppert M, Schmitz RA
Membrane association of Klebsiella pneumoniae NifL is affected by molecular oxygen and combined nitrogen.
Archives of Microbiology. 117: 223-234.

Klopprogge K, Stips J, Schmitz RA
The inhibitory form of NifL from Klebsiella  pneumoniae exhibits ATPase activity only when synthesized under nitrogen sufficiency.
Biochem. Biophys. Acta. 1594: 243-54.

Ehlers C, Grabbe R, Veit K, Schmitz RA
Characterization of GlnK1 from Methanosarcina mazei strain Gö1: Complementation of an Escherichia coli glnK mutant strain by M. mazei GlnK1.
J. Bacteriol. 184: 1028-1040.


Grabbe R, Kuhn A, Schmitz RA
Cloning, sequencing and characterization of Fnr from Klebsiella pneumoniae.
Antonie Van Leeuwenhoek. 79(3-4): 319-26.

Grabbe R, Klopprogge K, Schmitz RA.
Fnr Is required for NifL-dependent oxygen control of nif gene expression in Klebsiella pneumoniae.
J Bacteriol. 183(4): 1385-93.


Henne A, Schmitz RA, Bömeke M, Gottschalk G, Daniel R
Screening of environmental DNA libraries for the presence of genes conferring lipolytic activity on Escherichia coli.
Appl. Environ. Microbiol. 66: 3113-3116.


Henne A, Daniel R, Schmitz RA, Gottschalk, G
Construction of environmental DNA libraries in Escherichia coli and screening for the presence of genes conferring utilization of 4-hydroxybutyrate.
Appl. Environ. Microbiol. 65: 3901-3907.

Articles in German

Daniel R, Schmitz RA, Streit W (2002) aus Wattenmeer und Wüstensand.
Georgia Augusta. 1: 111-115.

additional Publications in PubMed