Regulation of nitrogen fixation in Klebsiella pneumoniae.
K. pneumoniae is able to reduce molecular nitrogen to ammonia under oxygen- and nitrogen-limiting growth conditions. Synthesis of the key enzyme nitrogenase is regulated in response to molecular oxygen and combined nitrogen by the two regulatory proteins NifA and NifL as well as the nitrogen sensory protein GlnK. Our research is focused on the characterization of the cellular signal perception and transduction of the oxygen and nitrogen signal towards NifL and NifA by genetic, biochemical and molecular biological methods. (Funded by the DFG)