Molecular and biochemical characterization of the nitrogen regulatory network and stress response in Methanosarcina mazei.

Methanosarcina mazeiArchaea represent the second domain of Prokarya. They are phylogenetically distant to Bacteria and share many features more similar to their eukaryotic than their bacterial counter parts (e.g. transcription and translation machineries). Working with the model archaeon Methanosarcina mazei, we study and aim to understand the overall regulatory network of the nitrogen metabolism and stress response. Besides classical genetic and biochemical approaches we achieve genome-wide transcription analysis using whole genome DNA microarrays and RNAseq approaches (high through put sequencing of cDNA libraries) to analyze individual regulatory networks and potential cross talks between different regulons. Besides regulation on the transciptional level by proteins, currently our main focus is to study the role of non-coding regulatory RNAs in stress response of M. mazei to understand the regulatory mechanisms of those small RNAs on the molecular level. (funded by the DFG)